Antibody screening for secreted proteins expressed in Pichia pastoris.
نویسندگان
چکیده
genes. Methods Enzymol. 185:60-89. 5.Switzer, R.C., C.R. Merril and S. Shifrin. 1979. A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels. Anal. Biochem. 98:231-237. 6.Tabor, S. and C.C. Richardson. 1985. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. USA 82:1074-1078. 7.Whelan K.F., E. Colleran and D.E. Taylor. 1995. Phage inhibition, colicin resistance, and tellurite resistance are encoded by a single cluster of genes on the IncHI2 plasmid R478. J. Bacteriol. 177:5016-5027.
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ورودعنوان ژورنال:
- BioTechniques
دوره 21 5 شماره
صفحات -
تاریخ انتشار 1996